Directed Evolution: Methods And Protocols (Methods In Molecular Biology, 2461)

This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.

• | Author: Andrew Currin, Neil Swainston
• | Publisher: Humana
• | Publication Date: Jun 23, 2023
• | Number of Pages: 289 pages
• | Language: English
• | Binding: Paperback
• | ISBN-10: 1071621548
• | ISBN-13: 9781071621547